منابع مشابه
The Mu enhancer is functionally asymmetric both in cis and in trans. Topological selectivity of Mu transposition is enhancer-independent.
Mu DNA transposition from a negatively supercoiled DNA substrate requires interaction of an enhancer element with the left (attL) and right (attR) ends of Mu. The orientation of the L and R ends with respect to each other (inverted) and with respect to the enhancer is normally inviolate. We show that when the enhancer is provided in trans as a linear fragment, the head to head orientation of th...
متن کاملQuality Control in Mu DNA Transposition
Transposons are segments of DNA encoding protein the resulting Strand Transfer Complex (STC, also called factors that move the DNA segment to new locations in Type 2 complex) , the 59 ends of Mu DNA are still joined the genome. They are ubiquitous among organisms and to old flanking DNA sequence, while the 39 ends of Mu play an important role in genome evolution, including DNA are joined to new...
متن کاملVisualizing Mu transposition: assembling the puzzle pieces.
Mobile genetic elements, once a curiosity of maize genetics, are now known to be remarkably prevalent in the genomes of organisms ranging from pathogenic bacteria to humans. The varied consequences of these elements include the alteration of gene expression and the spread of drug resistance genes among bacteria. Additionally, many viruses and bacteriophages that covalently integrate their own g...
متن کاملTransposition of bacteriophage Mu in the Legionnaires disease bacterium.
Legionnaires disease is an acute respiratory disease that is often fatal for immunocompromised patients. The causative agent of this disease, Legionella pneumophila, is a Gram-negative bacterium that is present in a variety of aquatic environments. L. pneumophila is a facultative intracellular parasite; it grows within human phagocytic cells and eventually causes their destruction. In contrast ...
متن کاملSNP discovery by mismatch-targeting of Mu transposition
Single nucleotide polymorphisms (SNPs) represent a valuable resource for the mapping of human disease genes and induced mutations in model organisms. SNPs may become the markers of choice also for population ecology and evolutionary studies, but their isolation for non-model organisms with unsequenced genomes is often difficult. Here, we describe a rapid and cost-effective strategy to isolate S...
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ژورنال
عنوان ژورنال: Journal of the Korea Society for Industrial and Applied Mathematics
سال: 2013
ISSN: 1226-9433
DOI: 10.12941/jksiam.2013.17.087